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Image Search Results
Journal: Renal Failure
Article Title: EIF2AK2 protein targeted activation of AIM2 -mediated PANoptosis promotes sepsis-induced acute kidney injury
doi: 10.1080/0886022X.2024.2403649
Figure Lengend Snippet: PANoptosis regulated sepsis-induced renal injury in mice. (A) Images of TUNEL staining and TUNEL-positive cells in mouse kidney tissue. (B) LDH kit was adopted to test cytotoxicity. (C) AIM2 induces PANoptosis. (D) Western blot was utilized to determine the levels of pyroptosis, apoptosis, and necroptosis-related proteins in renal tissue of mice. *** p < .001. n = 5 mice/group.
Article Snippet: LDH levels were assessed utilizing an
Techniques: TUNEL Assay, Staining, Western Blot
Journal: Renal Failure
Article Title: EIF2AK2 protein targeted activation of AIM2 -mediated PANoptosis promotes sepsis-induced acute kidney injury
doi: 10.1080/0886022X.2024.2403649
Figure Lengend Snippet: AIM2 mediated sepsis-induced renal injury by regulating PANoptosis. (A) Transfection efficiency of sh-AIM2 was assessed in HK-2 cells by RT-qPCR. Next, HK-2 cells were transfected with sh-AIM2 or sh-NC, and treated with LPS. (B) Western blot detection of AIM2 expression. (C) PI staining was used to detect cell death rate. (D) LDH kit was used to test cytotoxicity. (E) Western blot was performed to determine the levels of pyroptosis, apoptosis, necroptosis and PANoptosis-related proteins. Data are presented as mean ± SD of three independent experiments. ** p < .01 and *** p < .001.
Article Snippet: LDH levels were assessed utilizing an
Techniques: Transfection, Quantitative RT-PCR, Western Blot, Expressing, Staining
Journal: Renal Failure
Article Title: EIF2AK2 protein targeted activation of AIM2 -mediated PANoptosis promotes sepsis-induced acute kidney injury
doi: 10.1080/0886022X.2024.2403649
Figure Lengend Snippet: EIF2AK2 targeted activation of AIM2 mediated PANoptosis in sepsis-induced renal injury. HK-2 cells were transfected with sh-EIF2AK2 or OE-AIM2, and treated with LPS. (A) Western blot detection of EIF2AK2 and AIM2 expression. (B) PI staining was used to detect cell death rate. (C) LDH kit was used to test cytotoxicity. (D) Western blot was conducted to determine the levels of pyroptosis, apoptosis, necroptosis, and PANoptosis-related proteins. Values were expressed as mean ± SD of three separate determinations. * p < .05, ** p < .01, and *** p < .001.
Article Snippet: LDH levels were assessed utilizing an
Techniques: Activation Assay, Transfection, Western Blot, Expressing, Staining
Journal: Renal Failure
Article Title: EIF2AK2 protein targeted activation of AIM2 -mediated PANoptosis promotes sepsis-induced acute kidney injury
doi: 10.1080/0886022X.2024.2403649
Figure Lengend Snippet: Inhibition of AIM2-mediated PANoptosis alleviated sepsis-induced renal injury in mice. The mice were subjected to different groups: Sham group, CLP group, CLP + PBS group, and CLP + A151 (AIM2 inhibitor) group. (A) HE staining and PAS staining were used to detect the changes of renal tissue in mouse model. (B) Serum levels of Scr and BUN in mice were tested by kits. (C) AIM2 expression in renal tissue of mice was assessed with Western blot. (D) Images of TUNEL staining and TUNEL-positive cells in mouse kidney tissue. (E) LDH kit was adopted to test cytotoxicity. (F) Western blot was utilized to determine the levels of pyroptosis, apoptosis and necroptosis-related proteins in renal tissue of mice. * p < .05, ** p < .01, and *** p < .001. n = 5 mice/group.
Article Snippet: LDH levels were assessed utilizing an
Techniques: Inhibition, Staining, Expressing, Western Blot, TUNEL Assay